A pixel size can be specified manually or
it can be obtained from an image that contains an object of known size. In the
latter case, after selecting an image file, the dialog box shown in Figure 1 appears.
1: Obtaining the pixel size from an image.
order to compute the pixel size, delimit a segment in the
image and specify its length in the unit of measure selected.
From this data, BioVis3D can determine the pixel size by measuring the
segment in pixels and comparing it with the specified segment length.
by selecting a unit of measure and setting the field Segment Length
with the length of the segment to measure. Once this information is entered, click once at the starting point of the segment, and
then click at the end. After the first click and
before the second click, a black segment is drawn
while the mouse is moved (see Figure 1) to show in the screen the
segment being measured.
that the segment length is mandatory to compute the pixel size. If the image is clicked while this field is empty, a message
requesting a valid segment length, will appear.
the segment is delimited, the pixel size is computed and shown in
the field Resulting Pixel Size.